Physicochemical Properties of the Native, Zinc- and Manganese-Prepared Metalloprotease of Bacillus polymyxa

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Physiochemical properties of the native, zinc- and manganese-prepared metalloprotease of Bacillus polymyxa.

The neutral protease of Bacillus polymyxa had a broad pH optimum (6.0 to 7.2) for activity at 37 C. The enzyme was most stable at pH 5.6 to 5.8. The protease had an optimum temperature of 37 C and was quite thermostable up to 35 C, but at higher temperatures the stability decreased rapidly. The substrate specificity of the protease was similar to that of the neutral proteases of other members o...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1973

ISSN: 0003-6919

DOI: 10.1128/aem.26.2.191-195.1973